ELECTROPHYSIOLOGICAL AND NEUROCHEMICAL STUDIES OF THE MECHANISMS OF THE ANTICONVULSANT EFFECT OF THE NEW ORIGINAL COMPOUND GIZH-298
https://doi.org/10.17749/2077-8333.2016.8.3.019-026
Abstract
The aim of this study was to investigate the electrophysiological and neurochemical mechanisms of the anticonvulsant effect of a new original compound GIZH-298 and to define the leading structure as the target for influence compound. Materials and Methods. The partial (focal) and secondary generalized seizures were modeled by methods of creation a chronic epileptic focus that was caused by cobalt applique on the brain of rats. The liquid chromatography (HPLC) analysis used for neurochemical study of the effect GIZH-298. There was studied the effect on metabolism and quantity of biogenic amines in the brain structures of rats. Results. It was found that GIZH-298 at a dose of 60 mg / kg (i.p.) has a pronounced effect on the primary and especially secondary generalized epileptic foci in various brain structures with a primary influence on the cortex. GIZH-298 at a dose of 60 mg / kg caused a statistically significant increase in the content of serotonin and dopamine in the frontal cortex after 30 minutes after the administration and reduced the rate of metabolism of dopamine in the dorsal striatum. Conclusion. The anticonvulsant effect GIZH-298 is enhanced with increased of epileptic system, may be due to increased synthesis of serotonin and dopamine in the cortex, and decreased metabolism of the latter in the striatum.
About the Authors
S. A. Litvinova
Zakusov Institute of Pharmacology
Russian Federation
Russian Federation
Litvinova Svetlana Aleksandrovna – PhD, Psychopharmacology Laboratory Researcher.
Baltiiskaya ul., 8, Moscow, 125315, Russia. Tel.: +7(495)6012414. E-mail: sa_litvinova@mail.ru
T. A. Voronina
Zakusov Institute of Pharmacology
Russian Federation
Russian Federation
Voronina Tatyana Aleksandrovna – MD, professor, head of the Laboratory.
Baltiiskaya ul., 8, Moscow, 125315, Russia. Tel.: +74956012414. E-mail: voroninata38@gmail.com
V. S. Kudrin
Zakusov Institute of Pharmacology
Russian Federation
Russian Federation
Kudrin Vladimir Sergeevich – PhD, Head of the Laboratory of Pharmacology neurochemical.
Baltiiskaya ul., 8, Moscow, 125315, Russia. Tel.: +7(495)6012153. e-mail: kudrinvs@mail.ru
I. O. Gaydukov
Zakusov Institute of Pharmacology
Russian Federation
Russian Federation
Gaidukov Igor Olegovich – Post-graduate.
125315, Moscow, Baltiiskaya ul., 8. Tel.: +74956012414. E-mail: i_gaydukov@mail.ru
L. N. Nerobkova
Zakusov Institute of Pharmacology
Russian Federation
Russian Federation
Nerobkova Lyubov Nikolaevna – PhD., Senior Research Fellow.
Baltiiskaya ul., 8, Moscow, 125315, Russia. E-mail: Ln_Nerobkova@mail.ru
M. B. Pisclova
Zakusov Institute of Pharmacology
Russian Federation
Russian Federation
G. G. Avakyan
The Russian National Research Medical University named after N. I. Pirogov of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Avakyan Georgii Gagikovich – PhD, Department of Neurology, Neurosurgery and Medical Genetics.
Ul. Ostrovityanova, 1, Moscow, 117997, Russia. Tel.: +7(495)5316941. E-mail: avakyan_georgy@mail.ru
L. А. Zhmurenko
Zakusov Institute of Pharmacology
Russian Federation
Russian Federation
Zhmurenko Lyudmila Aleksandrovna – PhD.
Baltiiskaya ul., 8, Moscow, 125315, Russia
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Review
For citations:
Litvinova S.A., Voronina T.A., Kudrin V.S., Gaydukov I.O., Nerobkova L.N., Pisclova M.B., Avakyan G.G., Zhmurenko L.А. ELECTROPHYSIOLOGICAL AND NEUROCHEMICAL STUDIES OF THE MECHANISMS OF THE ANTICONVULSANT EFFECT OF THE NEW ORIGINAL COMPOUND GIZH-298. Epilepsy and paroxysmal conditions. 2016;8(3):19-26. (In Russ.) https://doi.org/10.17749/2077-8333.2016.8.3.019-026
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