Preview

Epilepsy and paroxysmal conditions

Advanced search

NON-INVASIVE ASSESSMENT OF ANTI-SEIZURE DRUGS AND THEIR ABILITY TO PREVENT EPILEPTIFORM CHANGES

https://doi.org/10.17749/2077-8333.2017.9.4.086-095

Full Text:

Abstract

The aim of the study is the assessment of possibility of using «Bioscope» device to detect the direction of changes in the integrative state of organism of rats under the influence of analeptic and convulsive agent of corazole, and known anticonvulsant medicaments the Phenobarbital, luminal, and diazepam. Materials and methods. Six series of experiments were performed with a 90-minute recording of integrative state of animals in the norm, after injection of corazol (50 mg/kg, subcutaneously), after injection of luminal (40 mg/kg, intraperitoneally) and diazepam (2 mg/kg, intraperitoneally), as well as after combined injection of luminal and corazole, diazepam and corazole. To record and analyze the signals of Bioscope device, a comprehensive software package was developed in the LabView software environment. Results. Signals of «Bioscope» device are changed differently after injection of corazole, luminal or diazepam. At the same time, among all used indicators, the spectral distribution of BB-intervals of «Bioscope» signals turned out to be the most sensitive to different experimental conditions. After injection of corazole, a pronounced peak in the frequency range of 0.3 osc/min is formed in the spectral distribution of inter-peak BB-intervals, which, perhaps, reflects the epileptiform state of the animal’s organism. After combined injection of luminal and corazole, as well as diazepam and corazole, the spectral distribution of inter-peak BB-intervals approaches the control type, which indicates the effectiveness of used anticonvulsants medicaments. Conclusion. The conducted research revealed the expediency of using the «Bioscope» device complex for rapid assessment of the type and dose of anticonvulsant medicaments for inerruption of epileptiform states of the organism.

About the Authors

R. G. Paronikyan
The Institute of fine organic chemistry named after A. L. Mnjoyan scientific-technological center of organic and pharmaceutical chemistry of NAS of Republic of Armenia
Armenia

Paronikyan Ruzanna Garnikovna – PhD, Associate Professor, Senior researcher.

Pr. Azatutyan, 26, Erevan, Armeniya, 0014


R. Sh. Sarkisyan
The Institute of Physiology after L.A. Orbeli NAS RA
Armenia

Sargisyan Rafik Shavarshovich – PhD, Head of the Laboratory of integrative biology.

Ul. br. Orbeli, 22, Yerevan, 0028


M. N. Avagyan
The Institute of Surgery Mikaelyan
Armenia

Avagyan Mkrtich Norairovich – MD, Associate Professor, Head of the Department of Rehabilitation and Traditional Medicine.

Ul. Аsratyana, 9, Yerevan, 0052


M. S. Grigoryan
The Institute of fine organic chemistry named after A. L. Mnjoyan scientific-technological center of organic and pharmaceutical chemistry of NAS of Republic of Armenia
Armenia

Grigoryan Marina Senikovna – Junior researcher.

Pr. Azatutyan, Yerevan, 0014


A. D. Paronikyan
American University of Armenia
Armenia

Paronikyan Armen Davidovich – Student at the Department of Programming.

Ul. Marshala Bagramyana, 40, Yerevan, 0019



References

1. Kazaryan S.A., Paronikyan R.G. Farma (in Russian). 2016; 13: 37-41.

2. Paronikyan E.G., Dashyan Sh. Sh., Paronikyan R.G., Dzhagatspanyan I.A., Nazaryan I.M., Akopyan A.G., Minasyan N.S. Bioorganicheskaya khimiya (in Russian). 2017; 43 (5): 563-571.

3. Grigoryan N.P., Markosyan A. I., Paronikyan R.G., Sukasyan R.S. Khimikofarmatsevticheskii zhurnal (in Russian). 2017; 51 (8): 3-8.

4. Hovakimyan A.A., Sirakanyan S.N., Geronikaki A., Spinelli D., Hakobyan E.K., Paronikyan R. New heterocyclic systems: pyridofuro[3,2-d]pyrido[1,2-a]pyrimidin7(8)-ones with anticonvulsant activity. 17th Hellenic Symposium on Medicinal Chemistry. Thessaloniki. 2017; p. 35.

5. Kazaryan S.A., Paronikyan R.G. DNAN RA. 2017; 117 (4): 328-332.

6. Paronikyan R.G. Epilepsiya i paroksizmal’nye sostoyaniya / Epilepsy and paroxysmal conditions (in Russian). 2017; 9 (3): 40-46. DOI: 10.17749/2077-8333.2017.9.3.040-046.

7. Mashkovskii M.D. Medicinal products [Lekarstvennye sredstva (in Russian)]. Moscow. 2006.

8. Draayer J.P., Grigoryan H.R., Sargsyan R.Sh., Ter-Grigoryan S.A. Systems and Methods For Investigation of Living Systems. US Patent Application 2007; 0149866 A1.

9. Sargsyan R.Sh., Gevorkyan A.S., Karamyan G.G., Vardanyan V. T., Manukyan A.M., Nikogosyan A.H. Bioscope: new sensor for remote evaluation of the physiological state of biological system. Proceedings of NATO ARW “Physical properties of nanosystems“, Springer. 2010; 303-314.

10. Sargsyan R.Sh., Karamyan G.G., Gevorkyan A.S., Quantum-Mechanical Channel of Interactions between Macroscopic Systems, AIP (American Institute of Physics) Conference Proceedings. 2010; 1232: 267-275.

11. Sarkissov G. T., Sargsyan R.Sh., Chubaryan F.A., Petrosyan R.A., Karapetyan L.M., Hakopyan N. E. Noninvasive assessment of functional state of rats at experimental trichinosis (Trichinella Spiralis). Medical Parasitology. 2010; 2: 19-21 (in Russian).

12. Danielyan I.A., Avetisyan L.G., Margaryan Sh.G., Davtyan T.G., Aradzhyan G.M., Mushegyan G.Kh., Dzhaginyan A.V., Sarkisyan R.Sh. On the possibility of early non-invasive diagnosis of skin cancer in white mice. Collection of materials of the II International Scientific Conference “Modern Society: Problems, Ideas, Innovations” [O vozmozhnosti rannei neinvazivnoi diagnostiki raka kozhi u belykh myshei. Sbornik materialov II Mezhdunarodnoi nauchnoi konferentsii «Sovremennoe obshchestvo: problemy, idei, innovatsii» (in Russian)]. Stavropol’. 2013; 6-10.

13. Jaghinyan A.V. Non-invasive monitoring of embryonic development of the chick embryo. National Academy of Sciences of RA «Electronic Journal of Natural Sciences». 2015; 2 (25): 41-44.

14. Mikhailov V.M. The variability of the rhythm of the heart. Experience of practical application of the method [Variabel’nost’ ritma serdtsa. Opyt prakticheskogo primeneniya metoda (in Russian)]. Ivanovo. 2000; 200 s.

15. Baevskii R.M., Ivanov G.G. et al. Vestnik aritmologii (in Russian). 2001; 24: 65-87.

16. Guidelines for the content and use of laboratory animals. The eighth edition. Trans. with English. Ed. I.V. Belozertseva, D.V. Blinov, M.S. Krasilshchikova [Rukovodstvo po soderzhaniyu i ispol’zovaniyu laboratornykh zhivotnykh. Vos’moe izdanie. Per. s angl. pod red. I.V. Belozertsevoi, D.V. Blinova, M.S. Krasil’shchikovoi (in Russian)]. Moscow: IRBIS. 2017.


For citation:


Paronikyan R.G., Sarkisyan R.S., Avagyan M.N., Grigoryan M.S., Paronikyan A.D. NON-INVASIVE ASSESSMENT OF ANTI-SEIZURE DRUGS AND THEIR ABILITY TO PREVENT EPILEPTIFORM CHANGES. Epilepsy and paroxysmal conditions. 2017;9(4):86-95. (In Russ.) https://doi.org/10.17749/2077-8333.2017.9.4.086-095

Views: 133


ISSN 2077-8333 (Print)
ISSN 2311-4088 (Online)