Comparative efficacy evaluation for various anticonvulsant drugs to restraint epileptiform states in rats
R. G. Paronikyan,
R. Sh. Sargsyan,
J. S. Sarkissyan,
G. G. Avakyan,
V. M. Avakyan,
V. R. Sargsyan,
A. V. Margaryan https://doi.org/10.17749/2077-8333/epi.par.con.2025.214
Abstract
Objective: development of a simple algorithm to comparatively evaluate effectiveness of various anticonvulsant drugs (ACDs) for restraining epileptiform states in rats.
Material and methods. The experiments were conducted with 120 white male rats weighing 180–220 g. Using Bioscope hardware complex, the standard indicators of animals’ condition were assessed after injection of various ACDs and after corazole was subcutaneously administered in ACD-treated rats. For a quantitative assessment of the animals’ condition, a spectrum of the original Bioscope signals was constructed to determine interpeak intervals of the oscillatory signals, calculate 16 statistical indicators for a set of successive interpeak intervals followed by constructing their spectrum.
Results. An algorithm based on analyzed bioscopic indicators of the animals’ condition was proposed. It was shown that lamotrigine at a dose of 25 mg/kg did not restrain convulsive states after corazole administration, and its conditional effectiveness score was zero. However, after increasing the dose of lamotrigine to 70 mg/kg, its total effectiveness score reached 3. The use of topiramate at a dose of 15 mg/kg turned out to be very effective (maximal 6 points). The high efficiency of using classical drugs luminal and diazepam for restraining convulsive states (6 and 5 points, respectively) was also noted.
Conclusion. The proposed analytical methodology is functional and allows identifying ACDs that can be used most effectively to relieve body epileptiform conditions.
About the Authors
R. G. Paronikyan
Mnjoyan Institute of Fine Organic Chemistry, Scientific Technological Center of Organic and Pharmaceutical Chemistry, National Academy of Sciences of the Republic of Armenia
Armenia
Armenia
Ruzanna G. Paronikyan, Dr. Sci. Biol., Accoc. Prof.
26 Azatutyan Ave., Yerevan 0014
R. Sh. Sargsyan
Orbeli Institute of Physiology
Armenia
Armenia
Rafik Sh. Sargsyan, Dr. Sci. Biol., Prof.
22 Orbeli Brothers Str., Yerevan 0028
J. S. Sarkissyan
Orbeli Institute of Physiology
Armenia
Armenia
John S. Sarkissyan, Dr. Sci. Biol., Prof.
22 Orbeli Brothers Str., Yerevan 0028
G. G. Avakyan
Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
Georgy G. Avakyan, Dr. Sci. Med., Assoc. Prof.
1 bldg 7 Ostrovityanov Str., Moscow 117513
V. M. Avakyan
Alt-Med Center for Rehabilitation and Traditional Medicine
Armenia
Armenia
Vahan M. Avagyan
3/15 Hakob Hakobyan Str., Yerevan 0054
V. R. Sargsyan
Orbeli Institute of Physiology
Armenia
Armenia
Vahram R. Sargsyan, PhD
22 Orbeli Brothers Str., Yerevan 0028
A. V. Margaryan
National Center for Disease Control and Prevention, “Reference Laboratory Center” Branch
Armenia
Armenia
Armine V. Margaryan, PhD
37 David Malyan Str., Yerevan 0096
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For citations:
Paronikyan R.G., Sargsyan R.Sh., Sarkissyan J.S., Avakyan G.G., Avakyan V.M., Sargsyan V.R., Margaryan A.V. Comparative efficacy evaluation for various anticonvulsant drugs to restraint epileptiform states in rats. Epilepsy and paroxysmal conditions. 2025;17(2):153-160. (In Russ.) https://doi.org/10.17749/2077-8333/epi.par.con.2025.214
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