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The role of glutamine synthetase expression and cystine/glutamate transporter (SLC7A11, xCT) in epilepsy pathogenesis of patients with supratentorial brain gliomas

https://doi.org/10.17749/2077-8333/epi.par.con.2022.118

Abstract

Background. Epileptic seizures represent one of the leading clinical manifestations of glial brain tumors that develop on average in 51% of cases. In gliomas, epileptogenesis is quite complex and multifactorial.

Objective: to study a role of the expressed glutamine synthetase enzyme, as well as cystine/glutamate transporter (xCT system, SLC7A11) in the pathogenesis of epilepsy developing in patients with cerebral gliomas.

Material and methods. The study included 32 patients with supratentorial gliomas. The average age of the disease onset (diagnosis) was 50.69±18.01 years. All patients underwent inpatient examination and treatment at the Neurosurgery and Nervous Diseases Clinics of Kirov Military Medical Academy from 2018 to 2020. In all cases, a biopsy of tumor tissue was collected. Histological and immunohistochemical studies were performed (expression of glutamine synthetase and cystine/ glutamate transporter (SLC7A11, xCT)).

Results. Significant (ANOVA p=0.027, Mann–Whitney U-test p=0.033) differences in the expression of cystine/glutamate transporter (xCT system, SLC7A11) were revealed, by showing the following median values (lower quartile; upper quartile): 50% (45; 75) in the group of patients with seizures, 40% (40; 50) in the seizure-free group. The expression level of glutamine synthetase did not significantly differ in the groups with and without seizures.

Conclusion. The data obtained confirmed that one of the pathogenetic mechanisms for epilepsy development in patients with gliomas was related to highly expressed cystine/glutamate transporter (xCT system, SLC7A11) and, as a consequence, an increase in the extracellular glutamate level.

About the Authors

M. Yu. Prokudin
Kirov Military Medical Academy
Russian Federation

Mikhail Yu. Prokudin – MD, PhD, Faculty Member, Chair of Nervous Diseases

Saint Petersburg, Russia

RSCI SPIN-code: 4021-4432



B. V. Martynov
Kirov Military Medical Academy
Russian Federation

Boris V. Martynov – Dr. Med. Sc., Associate Professor, Neurosurgeon

Saint Petersburg

RSCI SPIN-code: 9953-3997



A. I. Yakovenko
Kirov Military Medical Academy
Russian Federation

Andrey I. Yakovenko – MD, PhD, Head of Pathomorphological Department, Central Pathology Laboratory

Saint Petersburg

RSCI SPIN-code: 5937-8120.



I. V. Litvinenko
Kirov Military Medical Academy
Russian Federation

Igor V. Litvinenko – Dr. Med. Sc., Professor, Head of Chair of Nervous Diseases

Saint Petersburg

WoS ResearcherID: F-9120-2013;

Scopus Author ID: 35734354000;

RSCI SPIN-code: 6112-2792.



V. Yu. Lobzin
Kirov Military Medical Academy; Mechnikov North-Western State Medical University; Children's Research and Clinical Center for Infectious Diseases, FMBA of Russia
Russian Federation

Vladimir Yu. Lobzin – Dr. Med. Sc., Professor, Chair of Nervous Diseases; Professor, Chair of Neurology; Senior Researcher, Research Department of Neuroinfections and Organic Pathology of the Nervous System

Saint Petersburg

WoS ResearcherID: I-4819-2016;

Scopus Author ID: 57203881632;

RSCI SPIN-code: 7779-3569.



D. V. Svistov
Kirov Military Medical Academy;
Russian Federation

Dmitriy V. Svistov – MD, PhD, Associate Professor, Head of Chair of Neurosurgery; Chief Neurosurgeon of the Ministry of Defense of the Russian Federation

Saint Petersburg

RSCI SPIN-code: 3184-5590.



V. S. Chirskiy
Kirov Military Medical Academy
Russian Federation

Vadim S. Chirskiy – Dr. Med. Sc., Head of Chair of Pathological Anatomy; Head of Department, Central Pathology Laboratory of the Ministry of Defense of the Russian Federation

Saint Petersburg

Moscow, Russia

RSCI SPIN-code: 7295-3369.



K. A. Chemodakova
Kirov Military Medical Academy
Russian Federation

Ksenia A. Chemodakova – Neurosurgeon, Neurosurgery Clinic

Saint Petersburg

RSCI SPIN-code: 8425-1808.



O. А. Klitsenko
Mechnikov North-Western State Medical University
Russian Federation

Olga A. Klitsenko – PhD (Biol.), Associate Professor, Chair of Pedagogy, Philosophy and Law

Saint Petersburg

RSCI SPIN-code: 7354-3080.

 



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Review

For citations:


Prokudin M.Yu., Martynov B.V., Yakovenko A.I., Litvinenko I.V., Lobzin V.Yu., Svistov D.V., Chirskiy V.S., Chemodakova K.A., Klitsenko O.А. The role of glutamine synthetase expression and cystine/glutamate transporter (SLC7A11, xCT) in epilepsy pathogenesis of patients with supratentorial brain gliomas. Epilepsy and paroxysmal conditions. 2022;14(2):204-213. (In Russ.) https://doi.org/10.17749/2077-8333/epi.par.con.2022.118

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ISSN 2077-8333 (Print)
ISSN 2311-4088 (Online)